The dream of every patient or doctor
is to have a simple and exhaustive
laboratory test able to identify
a very large number of allergens.



Starting from 2000, on the wave of genomic arrays, protein arrays were built and, within a few years, released for commercialization. Thermofisher ImmunoCAP ISAC was the first, followed by others, such as MedAll, Microtest and Euroline strips. In 2017, MADx introduced ALEX, an innovative allergen array suitable for the description of the extended IgE profile.

AllerGenius is an expert system applied to ALEX to facilitate the interpretation of results.

The complexity of allergen arrays
Results from the ALEX array are transmitted to the server homing AllerGenius.
The system sorts the results, calculates derivate parameters and elaborates results starting from hundreds of rules.
At the end of this analysis, a report is produced generated and sent directly to the user.

ico matrice allergeni

What is an allergen array?
Hundreds of microscopic drops – each containing an allergen – are spotted onto a small surface: thus the laboratory test will behave like a single tube.

ico sistema esperto

What is an Expert System?
A subset of Artificial Intelligence where one or more experts give rules to a computer allowing it to answer questions like the expert(s) would do.

ico intelligenza artificiale

What is Artificial Intelligence?
According to Feigenbaum, the capacity of a machine to behave like a human, by answering questions.

What AllerGenius does


The complexity of molecular allergy confined the interpretation of these tests to a small number of highly skilled allergists.

AllerGenius was first presented in 2014 on the WAOJ as an interpretation support for ISAC. When ALEX became available, AllerGenius was upgraded by adding extract allergens to molecular components.

AllerGenius allows expert allergists to have powerful and updated help for the interpretation of allergens array for their patients. Less skilled allergists can benefit from the results of a modern technology associated to a complete and dedicated intepretation system.


AllerGenius & Alex


ALEX can be used as a second level diagnostics in allergic patients both in both the top-down (from clinic to the molecules) and in the bottom-up (from an extended IgE profile to the clinic and the therapy) approaches.

Results from the ALEX array are transmitted to the server housing AllerGenius. The system sorts the results, calculates derivate parameters and analyses results starting from hundreds of rules. At the end of this analysis, a report is produced and sent directly to the user.

AllerGenius, with its hundreds of rules, can sort allergens and components according to different families, identify the risk, verifying the consistency of the laboratory results, verifying whether the IgE profile is consistent with the patient’s clinic and, if required by the physician, AllerGenius can give first line therapeutic indications based on international guidelines.

Depending on the quality of the connection, this takes between 3 and 10 seconds.

Report example






Alex Array


ico esperti

Who are the experts in AllerGenius?
Hundreds of rules were written starting from the known literature, allergy databases, and the daily experience of molecular allergists.

ico livelli diagnostici

Which are the Diagnostic levels?
First Level: medical examination, Skin Prick Test.
Second Level:
Specific IgE assay for allergen sources.
Third Level:
Specific IgE assays for molecular allergens by Single-Plexed or Multi-Plexed (ALEX) techniques.
Forth Level:
Functional Assays, BAT, CAP-inhibition.

ico strategia diagnostica

Which are the two diagnostics strategies?
Top-Down: From medical examination thought the various levels (I, II,III, IV) up to the therapy.
Bottom-Up: From the extended IgE profile (ALEX) used as first screening to the therapy.

What is a Modular Expert System?
A system where the user can decide how to deepen explanations. In Allergenius this is achieved by
• The use of links to table explaining allergens, components, families, molecular structures, associated syndromes etc.
• The choice of insert in the report • a short summary
• A graphic representation of the results
• The list of all positive allergens and components
Therapy suggestions
• The list of possible pollen-food syndromes

AllerGenius: present and future


The AllerGenius version for ALEX was developed by Phenomix in 2017 and it is under continuous development.
At present (December 2018) the following international version of AllerGenius are available from Phenomix:

English, Italian, German, Spanish, French, Croatian, Romanian, Russian, Ukrainian

Under development are Czech version of Allergenius

• Other languages can be implemented upon request




Phenomix ltd is a young company located in Genova (Italy) and active in the field of:

• Artificial intelligence and expert systems.

• Nano- and micro technologies in medicine diagnostics.

• Development, support and quality controls of biological drugs for human use.

LPA supported the Allergenius project from the beginning by making available Flex 4800, then Flex 5000 and now Flex 6000, by building the API for Raptor and by improving the quality of the AllerGenius Code.

Logo Totalsolution

Totalsolution supported the Allergenius project from the beginning by excellent hardware support and helping in the implementation of the original web database.

MADx developed ALEX and allowed the link of AllerGenius to their instruments and results.

Adliance developed RAPTOR and collaborated from the ALEX side in the construction of the API for Allergenius .

Designed and built this web-site.

Supports the development and continuous update of AllerGenius and manages the use of AllerGenius in different laboratories and countries.